Testing for Peanut Allergens
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What is tested?
All received
ingredients:
- Cookie
Ingredients
- Brownie
Ingredients
- Baking
Mix Ingredients
- Baking
Powder Ingredients (Bulk and Super Sack)
- Cornstarch
- Soda
- MCP
- SAS
- SAPP
A sample
of each ingredient is taken upon arrival at the facility. The sample
must also be accompanied by a Certificate of Analysis (C of A) and all
paperwork must match.
The received
ingredient is not taken to its designated production floor until testing
is complete and shows less than 2.5 parts per million.
Every
cookie blend produced at Clabber Girl is also tested on site.
- One
tub from each finished production run is sent to an independent laboratory
for additional testing.
- An additional
finished tub from every production run is kept in an on-site library
at Clabber Girl.
- A test
is performed on one finished baking powder product, picked at random,
every week.
Testing Method
Neogen Veratox®
Allergen Testing
- Quantitative
results determine if an ingredient or product has been cross contaminated
with a peanut allergen.
Description
- 50
grams of a sample is ground to the consistency of finely ground
coffee.
- A
5g sample is taken from the ground sample.
- Warmed
extraction fluid and extraction additive are added to the sample.
- The
samples are shaken in a water bath for fifteen minutes.
- The
samples are removed from the water bath, and allowed to settle.
- Five
controls, along with each of the samples are transferred into red-marked
wells.
- Upon
completion, the contents of the wells are transferred into antibody-coated
wells and allowed to incubate for ten minutes.
- The
wells are then washed out using wash buffer. The wells are then
tapped out until the wash buffer is removed.
- Conjugate
is then added to each well, and allowed to incubate for ten minutes.
- The
wells are then washed out using wash buffer. The wells are then
tapped out until the wash buffer is removed.
- Substrate
is then added to each well, and allowed to incubate for ten minutes.
- Red
stop solution is then added to each well at a 45 degree angle.
- The
bottom of the wells are then wiped off and read in a well reader
using a 650nm filter.
- The
results for each sample are then printed out.
Vendor Statements
- All of
our vendors have filled out a form listing what allergens of the “big
eight” are present in their facilities.
- Most of
them do not contain peanuts, but there are a few who do have peanuts
in their facility.
- Vendors
with peanuts present in the manufacturing plant have stated on the designated
form that peanuts are not run on the same line or that they have effective
procedures to avoid cross contamination.
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